Antioxidant and anti-inflammatory effects in lipopolysaccharide-induced THP-1 cells of coumarins from the bark of Hesperethusa crenulata R.

Abstract All parts of Thanakha ( Hesperethusa crenulata R.) have been used as traditional skin care herbal material in Myanmar. In this study, coumarins from H. R. bark were isolated through solvent extraction, systematic fractionation, and repeated column chromatography. Spectroscopic analyses using ESI–MS, 1D NMR (1H 13C), 2D (gHSQC gHMBC), specific rotation, circular dichroism, IR spectrometry revealed three 2 R -7-hydroxy-8-(2,3-dihydroxy-3-methylbutyl)-coumarin (compound 1 ), peucedanol methylpeucedanol 3 which first tree. Antioxidant capacities decreased follows: compound > . Treatments lipopolysaccharide-induced THP-1 human monocytic cells with compounds at 378.8 μM 359.7 inhibited tumor necrosis factor-α production by approximately 32.7% 13.3%, respectively, compared the negative control. summary, these results suggest that extracts can be a potent antioxidant anti-inflammatory source for cosmetic ingredients.